Research

From left to right: Puget Sound Skills Center BioMedical Research and Global Health program students Maryan Farah, Samantha Johnson and Lul Abdinoor. Offered in collaboration with Seattle Children’s Research Institute, the course is the first-of-its kind at a Washington state Career and Technical Education school.

On the day On the Pulse visited the BioMedical Research and Global Health program at Highline Public Schools’ Puget Sound Skills Center (PSSC), the students were preparing to extract DNA from plant specimens in order to learn about a process used by scientists for studying DNA.

Instructor, Dr. Noelle Machnicki, reviewed the protocol, including a detailed description of lysis – a process the students would be using to break open the cells – and then sent them to their benches to get started.

Machnicki, a biologist with a doctorate degree, skilled educator and a member of the Science Education Department at Seattle Children’s Research Institute, was immediately drawn to the opportunity to teach the first-of-its-kind yearlong program offered in a partnership between Seattle Children’s and the PSSC.

“The program intends to create a strong foundation in biological sciences for high school juniors and seniors through extensive hands-on laboratory experience and other educational and leadership opportunities,” said Machnicki. “It provides research training beyond what a student would get in a typical high school science class.” Read full post »

As the countdown to 2018 begins, we can’t help but look back on all of the amazing stories from Seattle Children’s that inspired readers in 2017. With over 100 stories of hope, care and cures posted on our blog this year, here are the top seven most-read posts of 2017.

1. Novel Diet Therapy Helps Children With Crohn’s Disease and Ulcerative Colitis Reach Remission

Adelynne, with her mom here, was diagnosed with Crohn’s when she was 8 years old. With the help of a special diet, Adelynne has been in clinical remission for more than two years.

A first-of-its-kind-study led by Dr. David Suskind, a gastroenterologist at Seattle Children’s, published in the Journal of Clinical Gastroenterology, found a special diet called the specific carbohydrate diet (SCD) could bring pediatric patients with active Crohn’s and ulcerative colitis into clinical remission.

The findings support the use of SCD – a nutritionally balanced diet that removes grains, dairy, processed foods and sugars, except for honey – as a sole intervention to treat children with inflammatory bowel disease. Read full post »

Dr. Jane Buckner of the Benaroya Research Institute at Virginia Mason and Dr. David Rawlings at Seattle Children’s Research Institute are leading research to develop an immunotherapy for type 1 diabetes.

Advances in engineering T cells to treat cancer are paving the way for new immunotherapies targeted at autoimmune diseases, including type 1 diabetes. Now, researchers are also investigating therapies that reprogram T cells to “turn down” an immune response, which may hold promise for curing type 1 diabetes, as well as a number of diseases where overactive T cells attack a person’s healthy cells and organs.

“Instead of stimulating the immune system to seek and destroy cancer cells, treating autoimmune conditions will require programming a patient’s own T cells to tell rogue immune cells to calm down,” said Dr. David Rawlings, director of the Center for Immunity and Immunotherapies at Seattle Children’s Research Institute and chief of the Division of Immunology at Seattle Children’s Hospital.

Harnessing gene-editing techniques pioneered by Seattle Children’s, Rawlings and colleagues have already made headway in equipping T cells with the instructions needed to potentially reverse type 1 diabetes. In a new $2 million research project funded by The Leona M. and Harry B. Helmsley Charitable Trust, researchers will leverage these recent successes using this new form of T-cell immunotherapy into first-in-human clinical trials. Read full post »

Primary human B cells could offer the next promising cell therapy. Credit: Human B Lymphocyte by NIAID (CC by 2.0)

Scientists at Seattle Children’s Research Institute have unlocked the ability to engineer B cells, uncovering a potential new cell therapy that could someday prevent and cure disease.

In a paper published in Molecular Therapy, the research team describes how they genetically reprogrammed primary human B cells to act as cell factories capable of delivering sustained, high doses of therapeutic proteins. The gene editing techniques used reprogrammed B plasma cells to secrete a protein that could treat patients with hemophilia B.

Dr. Richard James, a principal investigator in the Center for Immunity and Immunotherapies at Seattle Children’s Research Institute and an author on the paper, discusses the significance of their discovery.   Read full post »

Genetic testing helped diagnose Nolan Wood, 3, with KCNQ3 epilepsy.

Even though Nolan Wood hadn’t experienced a seizure in more than two years, his parents still had questions about their son’s future.

“We wondered if there were others out there that have what Nolan has,” said Emily Wood, Nolan’s mom. “If so, what does their life look like?”

The Woods’ search for answers began when Nolan, 3, was diagnosed with infantile spasms and regression of his motor skills when he was 6 months old. Before receiving seizure medications, Nolan had hundreds of daily subtle, reflex-like seizures. Due to the regression of his motor skills, he had stopped rolling over, smiling and crying. A condition known as cortical visual impairment had also rendered him legally blind. Read full post »

It’s holiday time in the Louden household. However, this year is unlike any other. For the first time in 11 years, 17-year-old Amber Louden will be able to join her family at the Thanksgiving table and indulge in some of her favorite dishes pain-free.

“I remember Thanksgiving two years ago; I ate so much food that I ended up in the hospital because of the horrible pain I was in,” said Amber. “Last year, I didn’t even get a chance to sit at the dinner table because I spent the holiday in the hospital where I stayed for 12 days.”

Amber’s decade-long battle with chronic pancreatitis prevented her from partaking in cherished holiday traditions.

It may be surprising that these traditions and the root of Amber’s struggle with pancreatitis share one common factor — and that happens to be family.

Read full post »

Kaitlyn and Ryan Wyckoff travel from their hometown of Wasilla, Alaska, to Seattle Children’s so Dr. Sihoun Hahn (center) can monitor and treat them for Wilson disease — a rare genetic disorder.

For the first 15 years of his life, Ryan Wyckoff appeared to be a perfectly healthy, active teenager, living with his family in Wasilla, Alaska.

But during New Year’s weekend in 2009, Ryan began to feel seriously ill. He was lethargic and had a high fever that could not be controlled by acetaminophen.

Ryan was so sick he could barely make the trip to his family doctor. The doctor thought Ryan looked jaundiced and referred him to their local hospital, but providers there found nothing wrong so they sent him home.

Ryan’s symptoms worsened. He gained 15 pounds in just a couple days as fluid built up in his abdomen. Ryan’s mom, Lisa Wyckoff, remembered how her tall, slender son looked like he was pregnant.

An MRI revealed Ryan had cirrhosis — advanced scarring in his liver. His condition was life-threatening, so he was flown to Seattle Children’s by Medivac.

“It’s terrifying to have something seriously wrong with your son that no one can figure out,” said Lisa. “We felt so helpless.” Read full post »

Seattle Children’s researchers consulted with the Northwest Junior Football League before moving ahead with a CDC-funded program addressing safety and concussion awareness in youth sports. Photo courtesy of Brian Bodine Photography/NJFL

Seattle Children’s researchers will launch an innovative program in early 2018 aimed at shifting the culture of safety in youth sports and building concussion awareness during competitive play.

The program, called One Team, emphasizes community engagement in conducting brief pre-game safety huddles involving coaches, officials, parents and athletes, with a goal of addressing both sportsmanship and the importance of removing an athlete from play if they potentially have a concussion.

Dr. Sara Chrisman and Dr. Emily Kroshus, both members of the Seattle Pediatric Concussion Research Collaborative and Seattle Children’s Center for Childhood Health, Behavior and Development, designed the program.

“We want to change how children, parents and coaches relate to injuries, and reinforce a line in athlete safety that shouldn’t be crossed, even in a competitive atmosphere,” Chrisman said.

Read full post »

Group B streptococcus. Credit: NIAID. This work is licensed under a Creative Commons Attribution 4.0 International License.

Mast cells. Few know that mast cells are the first responders of the immune system. Even fewer study their role in group B streptococcus, a widespread bacterial infection that can cause preterm birth, stillbirth or dangerous sepsis in infants.

Many women who have given birth will likely remember the group B strep screening in their third trimester.

“Group B strep is normally found in up to 30% of women, but because it doesn’t cause illness, we don’t think about it until there is a risk in pregnancy,” said Dr. Lakshmi Rajagopal, a principal investigator in the Center for Global Infectious Disease Research at Seattle Children’s Research Institute. “In babies, the infection can lead to serious and deadly health consequences such as pneumonia, sepsis and meningitis.”

To ensure the infection is not transmitted to the baby, women testing positive for group B strep receive antibiotics during labor. However, no therapeutic strategies exist to prevent the incidence of systemic group B strep infection that can occur in babies before labor or after birth.

Rajagopal and Dr. Adrian Piliponsky, a principal investigator in the Center for Immunity and Immunotherapies at Seattle Children’s, believe part of the answer to preventing group B strep may lie in mast cells. In a study published in the Journal of Allergy and Clinical Immunology, the researchers reveal new insight into how mast cells defend against bacterial infections. Read full post »

Dr. Todd Cooper leads Seattle Children’s High-Risk Leukemia Program.

Seattle Children’s is getting set to launch a program that will redefine how we care for children with “high-risk” leukemia – or leukemia that doesn’t respond well to standard treatments and/or has relapsed after therapy.

Unfortunately, less than 40% of children with high-risk leukemia will live for more than four years after they’re diagnosed. Our new High-Risk Leukemia Program aims to cure more of these children by uniting their doctors onto one team, and by using state-of-the-art diagnostic tests to match kids with the latest treatments and clinical trials. The program will also partner with researchers to pursue new treatments and cures.

The first-of-its-kind program is expected to attract patients and families from across the country, and it’s being led by Dr. Todd Cooper as part of his lifelong mission to improve care for children with high-risk leukemia. On the Pulse sat down with Cooper to learn about how the new program will transform care and bring new hope to children and families. Read full post »